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ţƤʴCortisolELISAԼԭ
ʱ䣺2019-05-15   124

ţƤʴCortisolELISAԼԭ

 

ʵԭ

Լв˫һķø飨ELISAԤȰţƤʴCortisolİ΢Уμ걾׼ƷHRPǵļ⿹壬ϴӡõTMBɫTMBڹøĴ߻תɫתյĻɫɫdzƷеţƤʴCortisolءø450nm ²ⶨȣOD ֵƷŨȡ

 

  1.   ƽ20minȡʣԷܷŻ4档
  2.   ñ׼Ʒ׺ף׼Ʒ׸ӲͬŨȵı׼Ʒ50μL
  3.   50μLհ׿ײӡ
  4.   հ׿⣬׼Ʒ׺ÿ׼øHRPǵļ⿹100μL÷ĤסӦף37ˮԡ60min
  5.   ȥҺ壬ˮֽĸɣÿ׼ϴҺ350μL1min˦ȥϴҺˮֽĸɣظϴ5ΣҲϴϴ壩
  6.   ÿ׼AB50μL37ܹ15min
  7.   ÿ׼ֹҺ50μL15minڣ450nmⶨ׵ODֵ

 

 

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